Notes

This test is New York DOH approved.

Collect

2 mL blood in EDTA (Lavender)

Minimum Collection Volume

1 mL EDTA (Lavender)

Unacceptable Conditions

Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Storage/Transport Temperature

AMBIENT

Specimen Preparation

Keep specimen as whole blood at room temperature. Specimen must be received in lab within 24 hours of collection.

Stability (from collection to initiation)

Ambient: 24 hours / Frozen: Unacceptable / Refrigerated: Unacceptable.

Performed

Mon- Fri (Specimen must be in the CBDI lab by 11am on Fridays)

Performing Lab

CBDI DIL 513-636-4685

Units of Measure

%

Methodology

Flow Cytometry

Reported

2 days

Tests Included

Intracellular expression of Perforin and Granzyme B in NK cells and CD8+CD57+ cells.

Synonyms

  • HLH: Perforin/Granzyme B by flow
  • Granzyme B

Interpretive Data

This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high.

CPT Codes

88184, 88185x4, 88187
Collection

Notes

This test is New York DOH approved.

Collect

2 mL blood in EDTA (Lavender)

Minimum Collection Volume

1 mL EDTA (Lavender)

Unacceptable Conditions

Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Storage/Transport Temperature

AMBIENT

Specimen Preparation

Keep specimen as whole blood at room temperature. Specimen must be received in lab within 24 hours of collection.

Stability (from collection to initiation)

Ambient: 24 hours / Frozen: Unacceptable / Refrigerated: Unacceptable.

Performed

Mon- Fri (Specimen must be in the CBDI lab by 11am on Fridays)

Performing Lab

CBDI DIL 513-636-4685

Units of Measure

%
Ordering

Methodology

Flow Cytometry

Reported

2 days

Tests Included

Intracellular expression of Perforin and Granzyme B in NK cells and CD8+CD57+ cells.

Synonyms

  • HLH: Perforin/Granzyme B by flow
  • Granzyme B
Result Interpretation

Interpretive Data

This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high.
Laboratory Personnel Use

CPT Codes

88184, 88185x4, 88187